SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. Acridine orange (AO) staining evaluates the morphological changes in apoptotic cells by formation of blebs in the membrane, fragmentation and condensation of the. Each well was scanned at nine evenly distributed points and averaged automatically with the software. . The sample absorbance was read at 450 nm using a SpectraMax M4 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. Finally, the optical density was evaluated using SpectraMax M4 plate reader (Molecular Devices, San Jose, CA, USA) at 450 nm. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading 312 gain calibration failed. In vitro transcription (IVT) is an essential technique for RNA synthesis. 23, SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 23 PathCheck Pathlength Measurement Technology and Interfering Substances Any material that absorbs in the 900 nm to 1000 nm spectral region could interfere with PathCheck Pathlength Measurement Technology measurements. 次いで、ユーザーのラボに. VIEW DETAILS The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. , cyan fluorescence at 458 ex. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. 2. Plates were then read at 450 nm using a SpectraMax M4 device (Molecular Devices, Sunnyvale, CA). 4) In vivo pharmacokinetic studies. Background-subtracted data values (mean +/- SD) are graphed. Fluorescence Microplate Reader Comparison Chart. We therefore gathered salient. MIC assay: After diluting colloidal nanoparticle solutions of varying concentrations in TSB, they were injected onto a 96-well plate with a strain of bacteria at a concentration of 10 6 CFU/mL. Brand: Molecular. qPCR The jejunum was sampled immediately after termination, snap frozen, andChoose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. . For more detailed information and technical support of Molecular Devices instruments or software,Luciferase Detection Instrumentation Guide. SpectraMax® M5. The SpectraMax M5e reader offers the additional benefit of being certified . Cells were stained with dichlorofluorescein diacetate, fixed, and immediately analyzed using a multianalytic validation system (SpectraMax M4). Indie Digital & Print Publishing. SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. SpectraMax® Paradigm® Multi-Mode Detection Platform. Eos - M4/3 lens adapter - Lenses - Victoria, British Columbia | Facebook. N2a cells were induced for neurite growth by adding nerve growth factor (NGF) (50 ng/ml). The IL-10 standard curve was prepared as described in Section 10. alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. 4. book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. Because of possible losses from settling, particle data for the >7 µm size fraction (<0. 3. Steady-state kinetics measurements were performed at 25°C in 96-well plates (Corning) using a SpectraMax M4 spectrophotometer (Molecular Devices) in PBS buffer. Molecular Devices SpectraMax M4 User Manual (86 pages) Multi-Mode Microplate Readers. 1. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. To quantify F-CHP fluorescence, stained samples were digested in 500 µl of 1 mg/ml proteinase K in water for 3 hours at 60 °C. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of. Background-subtracted data values (mean +/- SD) are graphed. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers;. Then the formazan crystals were solubilized in dimethyl sulfoxide, and the absorbance was recorded at 572 nm wavelength using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). 0 calibration logic, which needs only 5 minutes and a single sample per day rather than the 30 or more minutes needed by conventional analyzers. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). For cell cultures, the medium was removed 24 h after initiation of drug stimulation and frozen for subsequent analysis, with no need for sample dilution. The absorbance was measured spectrophotometrically using a microplate reader (SpectraMax M4, Molecular Devices, CA, USA) at 570 nm. View specifications, prices, citations, reviews, and more. 2. l. item 3 replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon. The optical absorbance of the plate at 450 nm was measured using a multifunctional microplate reader SpectraMax M4 (Molecular Devices, LLC, San Jose, CA, USA). A series of Toll-like receptor 7 (TLR7)-specific antagonists and extensive structural analysis reveal the open conformation of the receptor and the structural basis of TLR7 antagonism. 9000-0133 SpectraMax®MousePad 9000-0972 Packageof5samplemicroplates 2400-2464 DustCover 5000505 CEDeclarationofConformity 5019680 Card,UserGuideDownload The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Table 2. Choose cables that meet the requirement described in this appendix. . /525 em. Tris solution and the fluorescence of the solution was measured using a fluorometer (SpectraMax M4, Molecular Devices). FlexStation ®, SpectraMax. SpectraMax M2/M2e reader: TRUE: Molecular Devices: Aquamax DW4 washer: TRUE: Molecular Devices: SpectraMax M4 reader: TRUE: Molecular Devices: SpectraMax M5/M5e reader: TRUE: Molecular Devices:. Cleavage of the fluorescent NAG substrate (4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside) was measured for 20 min with 45 s intervals at 30°C in a Molecular Devices (San Jose, CA) SpectraMax M4 microplate reader. 2. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Results were expressed as a percentage relative to the control group (defined as 100%). If using the StakMax and a SpectraMax reader together, the time specifications for the movement of the. The instrument reads all Plate sections in the experiment. All associated StakMax® stackers. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax® M4 Microplate Reader Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. 39. for Cisbio Bioassays. We have 2 Molecular Devices SpectraMax M4 manuals available for free PDF download: User Manual . To investigate the pharmacokinetics of HSA–CBT-NPs, 10 male Sprague-Dawley rats (mean±SD=250±20A SpectraMax M4 microplate reader was purchased from MeiGu Molecular Co. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. Bioz Stars score: 98/100, based on 1 PubMed citations. Detection modalities are shown in Table 1-1. Interpolated concentrations of native CRP in human serum and plasma samples. To determine the cell viability, thiazolyl blue tetrazolium bromide (MTT) assay was performed. ISM Equipment Classification (Group 1, Class A) This equipment is. SpectraMax M4 Multi-Mode Microplate Reader. Background-subtracted data values (mean +/- SD) are graphed. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. 73. Data analysis methods. Hemoglobin release from erythrocyte lysis was detected by measuring the optical density (OD) of the supernatant at 412 nm using a microplate reader (SpectraMax M4 from Molecular Devices, or Tecan SPARK, from Tecan, Männedorf, Switzerland). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. 161 likes. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. The optical density at 450 nm was detected using a microplate reader (SpectraMax M4, MolecularDevices, CA, USA). Table of Contents. SpectraMax® M4). "With this complete portfolio of multi-mode configurations, we can deliver multi-mode. . The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. Table 1. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. The conjugate was also characterized using FTIR (FT/IR-4200, Jasco Inc. All configurations. Published Dec 16, 2020 | Updated Dec 16, 2020. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. S. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Molecular Devices. 3 or greater. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. In this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. $1,250. The intestinal barrier has been recognized as being of major relevance for a number of diseases, namely digestive diseases like inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), and celiac disease (CD), but also extra-intestinal diseases, such as asthma, arthritis, and the metabolic syndrome [1–4]. SpectraMax M3 Multi-Mode Microplate Reader with 1-Year Service Contract. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 383 inches whereas the maximum allowed is 5. The content was vortexed for 1 min and incubated in the dark for 20 min at 25°C. Fifteen different automated tests are provided: • Background noise • Background spike • Lower limit of detection • Crosstalk • Linearity • Relative luminescence units (RLU) • Kinetic noise (low signal)0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 306 plate air cal fail,low light. StakMax Handling Stacker Base Unit. The MTT reagent was incubated with cells for 4 h. F1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. Quick, Simple Standardization: The new analyzer features SPECTRO’s proprietary iCAL 2. The following document is intended to demonstrate setup of this instrument. For the quantification of the number of viable bacteria in the biofilm, the biofilms were gently destroyed and plated on TSA after serial. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. Recertification is recommended every two years. Five hours prior to when permeability will be measured, gavage mice with 0. ). Lower limits of detection for ATP determined using the ATPlite . Bottom-reading is usually recommended for cell-based assay with adherent cells. ZERO BIAS - scores, article reviews, protocol conditions and more7. $2,219. Choose from a three- (M3), four- (M4), orCables. spr SpectraMax Plus 384. The absorbance of solubilized formazan crystals was measured at 570 nm using a multimode reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, CA, USA). The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence, and can be upgraded to include fluorescence polarization. normalized response (Graphpad Prism 8. ROS estimation by 2′,7′- dichlorofluorescin diacetate (DCFDA) staining . 7. The luciferase values were determined by normalization to Renilla values (as a control) to correct for variation in transfection efficiencies. Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. com. For more detailed information and technical support of Molecular Devices instruments or software, Luciferase Detection Instrumentation Guide. Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax. Transfer plate to SpectraMax M5 with excitation monochromator set to 455 nm with 10 nm bandwidth, emission monochromator set to 525 nm with 10 nm bandwidth, and 515 nm cutoff filter. There are multiple vendors. Images were taken using a fluorescence microscope (Nikon 80i, Japan). Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Dumont3, Matthias S. StakMax Handling Stacker Base Unit. Then, MTT (10 μl of 5 mg/ml) was added to each well and further incubated for 4 h. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The fluorescent signal was measured by multiplate reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, California, USA). All configurations. e. Results and Discussion Small-molecule agonists have been identified for TLR7 [ 41 , 42 , 47 , 48 , 60 – 67 ], TLR8 [ 49 , 51 – 54 , 68 ], TLR4 [ 69 – 72 ] and TLR2 [ 73 , 74 ]. The UV‐Vis absorption spectra and cell viability were measured by a multi‐mode microplate reader (SpectraMax® M4, Molecular Devices LLC) using quartz cuvettes with 1‐cm path length (Starna Scientific Ltd. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable Monochromator UV/Vis absorbance Fluorescence intensity, FRET, TRF, TR-FRET and Luminescence as primary modes Tunable. Three kits covering a wide range of dsDNA concentrations. All of the experiments were performed in triplicate, and the results were represented as mean. alamarBlue reagent volume in a different spheroid microplate formatAfter incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). 0 statistics software. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Laboratory Freezers. 1 & 7. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Where is the instrument serial number located? The serial number is on. A microplate reader (SpectraMax M4; Molecular Devices) was used to measure the absorbance (Abs) at 545 mM and the hemolysis rate was calculated as follows: (Eq. Next to the pushbutton is a green LED. TYPICAL SAMPLE VALUES Sensitivity: The calculated minimal detectable dose (MDD) is 15. 2. Microplate Readers include the M3, M4,. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeThen, cells were stained with MitoSox Red (5 μM) for 30 min. The percentage of viability of each treatment group in different. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. The IC50 curves were calculated using variable slope for log (inhibitor) vs. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. Cells were seeded at 5000 cells well −1 on 96-well plates and allowed to grow for 24 h before treatment. After incubation, the fluorescence intensity was measured using Spectramax M4 (Molecular Devices) (excitation: 380 nm emission: 460 nm). (LMax II, SpectraMax L), with a Lower Limits of Detection (LLD) use six different light intensities for Detection Estimates, Magnitude 1 through 6. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. 1. Examples: “Press carefully to engage,” “ Do not. Choose from a three- (M3), four- (M4), orLuciferase Detection Instrumentation Guide. C. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. org) with optimized instrument settings (Table 1) were used to generate and analyze data. Note: In this user guide, SpectraMax ® refers to several SpectraMax . We therefore gathered salient information about many of the. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. DNA standards. The medium was then removed and 100 μL of DMSO was added to dissolve the formazan crystal. 11. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Cellular viability was assessed by using resazurin at 1. WARNING! The instrument weighs approximately 36 pounds (16. 7. 05 mg mL −1. Cell viability (%) was calculated as the ratio of the number of living cells in treated samples to that of the control [15,30]. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. In. • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderThe medium was replaced with CM from the macrophages of the different groups and then incubated for 48 or 72 h. Choose from a three- (M3), four- (M4), or Qualify the luminescence performance of the SpectraMax . The BD plate dimensions are 5. Serum C-reactive protein (CRP) was quantified using the Mouse C-Reactive Protein/CRP Quantikine ELISA Kit. When a feature or capability applies to only certain readers, this exception is noted. to View Figures. Real time thermal cyclers (iCycleriQ apparatus with iCyclerOptical System software version 3. 50 μl of samples were added into the mixture of 40 μl of detection buffer and 10 μl of Ac-DEVD-pNA (2 mM) with gentle shaking for 5 min. Page 17 Chapter 2: Set Up the StakMax Microplate Handler Microplate Handler Mounted Baseplate Connection This procedure provides instructions to connect the microplate handler to the following instruments: Gemini EM Gemini XPS SpectraMax M2 SpectraMax M2e SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e SpectraMax. Description 1. . When a feature or capability applies to only certain readers, this exception is noted. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. 2 only) SpectraMax M-series Microplate readers. Dettmer4, Wayne A. Data are presented as mean values ± standard. 5 mg mL −1 with 0. For labeling representative cells, they were treated under the same conditions as above, followed by fixation with 2% paraformaldehyde for 20 min at room temperature. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. All experiments. Background-subtracted data values (mean +/- SD) are graphed. However, little is known about the regulatory mechanisms of the degradative pathway. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate Reader The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Add To My List; Create New List; Quickview. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. 3. 304 signal level saturation. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. • SpectraMax® Paradigm (multi-mode) • SpectraMax® M5e / M5 (multi-mode) • SpectraMax® M4 / M3 (multi-mode) • SpectraMax® M2e / M2 (dual mode) • Gemini ™ EM and Gemini ™ XPS (fluorescence) • SpectraMax® L • SpectraMax® Plus384 (serial number # -MNR-xxxxx)The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. Table 2. Cytosolic [K +] was then monitored using a SpectraMax M4 spectrofluorometer (San Jose, CA, USA) by loading AM at 37 ℃ for 40 min with 12 µM APG-2. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Ltd. , USA). Using cross-feeding bioassay, a functional LuxI/LuxR-type acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) system. Compare plate readers7 Chapter 1: Installation and Setup SoftMax Pro Microplate Data Acquisition and Analysis Software controls Molecular Devices spectrophotometers, absorbance, luminescence, and fluorescence microplate readers, and the SpectraMax Paradigm Multi-Mode Detection Platform. org) with optimized instrument settings (Table 1) were used to generate and analyze data. Choose from a three- (M3), four- (M4), orThe optical density was determined spectrophotometrically at 405 nm using a SpectraMax M4 microplate reader (Molecular Devices, Sunnyvale, CA). The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Description 1. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. ® reader to the computer. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. 4 kilograms). com. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. 7. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 2. The combination index (CI) was calculated. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader 5 B > 4 4 5 B 5 : F 8 8 > 3 ; > I 5 = 8 5. SpectraMax Gemini XPS/EM Register or Sign In. Example: [Drawer]. Pierce™ BCA Protein Assay Kit was purchased from Thermo Fisher Scientific Inc. Bioz Stars score: 98/100, based on 1 PubMed citations. Simulated Exposure SystemOptical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). Serum C-reactive protein (CRP) was quantified using the Mouse C-Reactive Protein/CRP Quantikine ELISA Kit. In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. Fluoresbrite® fluorescent nanoparticles were analyzed by spectrofluorimetry where particles were excited at 441 nm wavelengths and emission intensity was recorded at 486 nm wavelengths. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. 2. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. FlexStation™ III. item 7 replacement bulb for molecular devices spectramax m4 flash lamp replacement bulb for molecular devices spectramax m4 flash lamp. Diclofenac potassium at non-toxic concentration on U87-MG was used as positive control. 2. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e reader. Examples: “Press carefully to engage,” “ Do not press down. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. ELISA standard curves were generated using a four-parameter logistic curve model (GraphPad Prism 8, GraphPad Software; San Diego, CA, USA). These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. My List. Results. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. All experiments. Our absorbance plate readers feature our PathCheck Sensor. spr SpectraMax iD5 SpectraTest ABS2 v1. Instruments Ltd. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Flasks containing 100 mL of YM broth were inoculated with 100. Bio-layer interferometry assays The binding kinetics of 1H1 to spike or RBD proteins from SARS-CoV-2 variants was performed by biolayer interferometry analysis on the GatorPrime Label-Free Bioanalysis. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Find M4 Group Marketing Specialists in Victoria, with phone, website, address, opening hours and contact info. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. , 2020b). 2. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] number of moles of FITC conjugated to OVA was determined using UV–vis spectroscopy (SpectraMax M4, Molecular Devices Inc. STAKMAX. Transfected cells were seeded into 6-well plates at a density of 200 cells/well. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. Record fluorescence emission data in kinetic mode for 2 min with 20 s between reads to ensure that interaction has reached equilibrium and take average. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. No. Statistical analysis. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. Other technologies such as imaging. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. In the M2, M5 and Plus 384, the light. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. 5 mL of creatinine, FD-4, and rhodamine70 dissolved in. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. 2. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). For the microspheres containing moxifloxacin used in vivo, the liquid hydrogel was presoaked in a 24 mg/mL aqueous solution of moxifloxacin hydrochloride. 7°C and the bottom to 36. 4. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. To report an issue with this product, Brief content visible, double tap to read full content. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax® ABS. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. SPECTRAMAX 190 reader: TRUE: Molecular Devices: SPECTRAMAX 340PC/340PC 384 reader: TRUE: Molecular Devices. , and red fluorescence at 543 ex. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. 303 unable to cal dark current. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. For SpectraMax L or LMax II, LMax II. 116 SpectraMax M5 and M5e Multi-Mode. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader MTT reduction - a tetrazolium-based colorimetric assay for cell survival and proliferation (Maxline Application Note #5) (PDF) Summary: The MTT assay measures cell respiration and the amount of formazan. 2. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. Polybead® nanoparticles were analyzed. 6 mm). 9000-0756. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 38. , San Jose, CA) was. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader Where can I find UV/Vis Plastic Disposable Cuvettes? Some Plastic Disposable UV (300 nm +) and VIS Cuvette sources: NOTE: Some disposables with channel sides (as opposed to full. 116 SpectraMax M5 and M5e Multi-Mode. 5. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Other technologies such as imaging. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Standard features include a cuvette port,. Choose from a three- (M3), four- (M4), or Cables. The IC50 curves were calculated using variable slope for log (inhibitor) vs. 2. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Example: [Drawer]. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The plate reader optics are tunable for absorbance. 05 mol/L hydrochloric acid and 0. Plot the fluorescence intensity versus the cell seeding number. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates LM1 , FlexStation 3. Microplate Reader Shelf. Background-subtracted data values (mean +/- SD) are graphed. Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. The plate reader. The chamber contains springs that position the cuvette in the proper alignment for a read. Cell viability was calculated using the following formulaThe luciferase signal was read in a plate reader (SpectraMax M4; Molecular Devices, Sunnyvale, CA). For heme titration experiments a constant ATP concentration of 1 mM was used. Phillips5, Martin A. 5.